NAME: 	pENTR221 
RESISTANT MARKER:	Kanamycin resistant; 10 µg/ml
SOURCE: 	Invitrogen Life Technologies
V_TYPE: 	Gateway entry vector
SEQUENCING PRIMERS: 	M13(-21), M13 reverse
POLYLINKER SEQUENCE: 
cgacaaacaacagataaaacgaaaggcccagtcttccgactgagcctttc
gttttatttgatgcctggcagttccctactctcgcgttaacgctagcatg
gatgttttcccagtcacgacgttgtaaaacgacggccagtcttaagctcg
ggccccaaataatgattttattttgactgatagtgacctgttcgttgcaa
caaattgatgagcaatgcttttttataatgccaactttgtacaaaaaagc
aggct-LINKER-ORF-LINKER-acccagctttcttgtacaaagttggc
attataagaaagcattgcttatcaatttgttgcaacgaacaggtcactat
cagtcaaaataaaatcattatttgccatccagctgatatcccctatagtg
agtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtct
caaaatctctgatgttacattgcacaagataaaaatatatcatcatgaac
aataaaactgtctgcttacataaacagtaatacaaggggtgttatgagcc
atattcaacgggaaacgtcgaggccgcgattaaattcca

Note the specific antibiotic to be used with this vector.
Confirm sequencing primer sequences match vector before sequencing.

All ORF clones are provided in Entry Vectors for the Invitrogen Gateway® 
cloning system. Invitrogen offers a whole range of Destination Vectors for 
a wide range of applications. Refer to the homepage of Invitrogen for further 
information on the GatewayR cloning system and related Invitrogen products at:
<a href="http://www.invitrogen.com/content.cfm?pageid=4072">http://www.invitrogen.com/content.cfm?pageid=4072</a>
For further information on the ORFeome Collaboration, visit their homepage at 
<a href="http://www.orfeomecollaboration.org/html/index.shtml">http://www.orfeomecollaboration.org/html/index.shtml</a>.
For further technical information visit our homepage at: <a href="http://www.dnaform.jp">http://www.dnaform.jp</a> 
or contact us under: <a href="mailto:techinfo@dnaform.jp">techinfo@dnaform.jp</a>.