Vector Name: 	pFLCIII
Original Vector: 	pBluescript KS(+)
1st Primer for cDNA library construction: 	1st-BS primer (1st-BG tag)
2nd Primer for cDNA library construction: 	2nd-NX/X primer
Cloning Site (5'>3'): 	SalI/XhoI, SstI, BamHI
Sequence Primer (Fwd, 5'): 	M13-21
Sequence Primer (Rev, 3'): 	1233
Selection marker:	100 µg/ml Ampicillin

cDNA inserts can be release by digestion with I-CeuI and PI-SceI.
Those are Homing Endonucleases that commonly do not cut within cDNA inserts.
Note, the SalI and XhoI sites had been destroyed during cloning.