Vector Name: pFLCIII Original Vector: pBluescript KS(+) 1st Primer for cDNA library construction: 1st-BS primer 2nd Primer for cDNA library construction: 2nd-hairpin-linker primer Cloning Site (5'>3'): SalI/XhoI, SstI, BamHI Sequence Primer (Fwd, 5'): M13-21 Sequence Primer (Rev, 3'): (1233) Selection marker: 100 µg/ml Ampicillin cDNA inserts can be release by digestion with I-CeuI and PI-SceI. Those are Homing Endonucleases that commonly do not cut within cDNA inserts. Note, the SalI and XhoI sites had been destroyed during cloning.