Vector Name: 	pFLCIII
Original Vector: 	pBluescript SK(+)
1st Primer for cDNA library construction: 	1st-BS primer (1st-BG tag)
2nd Primer for cDNA library construction: 	2nd-linker primer
Cloning Site (5'>3'): 	SalI/XhoI, SstI, BamHI 
Sequence Primer (Fwd, 5'): 	M13-21
Sequence Primer (Rev, 3'): 	1233
Selection marker:	100 µg/ml Ampicillin

cDNA inserts can be release by digestion with I-CeuI and PI-SceI.
Those are Homing Endonucleases that commonly do not cut within cDNA inserts.
Note, the SalI and XhoI sites had been destroyed during cloning.
It had been noticed that in some clones from this services the"SalI/XhoI" site has been duplicated.
Since the "SalI/XhoI" site cannot be cut, this does not effect the use of the clone.