Vector Name: pFLCII Original Vector: pBluescript SK(+) 1st Primer for cDNA library construction: 1st-BG primer (short poly-A) 2nd Primer for cDNA library construction: 2nd-linker primer (g-tail less) Cloning Site (5'>3'): XhoI, BamHI Sequence Primer (Fwd, 5'): M13-21 Sequence Primer (Rev, 3'): FLCP2 Selection marker: 100 µg/ml Ampicillin Vector contains AttB1 and AttB2 recombination sites for Invitrogen Gateway System. cDNA inserts can be release by digestion with XhoI and BamHI. Check cDNA sequence before digestion for XhoI and BamHI sites to avoid internal cleavage.