NAME: 	pENTR223.1 
RESISTANT MARKER:	Spectinomycin resistant; 100 ��g/ml
SOURCE: 	Invitrogen Life Technologies
V_TYPE: 	Gateway entry vector
SEQUENCING PRIMERS: 	M13(-21), T7
POLYLINKER SEQUENCE: 
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCAGAAG-linker-ORF-linker-GCCCAGCTTTCTTGTAC
AAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACA
GGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCC
CCTATAGTGAGTCGTATTACATGGTCAT

Note the specific antibiotic to be used with this vector.
Confirm sequencing primer sequences match vector before sequencing.

All ORF clones are provided in Entry Vectors for the Invitrogen GatewayR cloning system. Invitrogen offers a whole range of Destination Vectors for a wide range of applications. Refer to the homepage of Invitrogen for further information on the GatewayR cloning system and related Invitrogen products at:
http://www.invitrogen.com/content.cfm?pageid=4072 
For further information on the ORFeome Collaboration, visit their homepage at http://www.orfeomecollaboration.org/html/index.shtml.
For further technical information visit our homepage at: http://www.dnaform.jp or contact us under: techinfo@dnaform.jp.