Vector: pOTB7 | Source: MGC Gateway | Collection: MGC | Download PDF Document |
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Name: pOTB7 Source: UCBerkeley/Rubin Vector type: plasmid Selection marker: Chloramphenicol resistant; 25 µg/ml Polylinker sequence: cgttagaacgcggctacaattaatacataaccttatgtatcatacacata cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc gaggcctgggtggcgaattcccttactagtttccctcgaggcatctatgt cgggtgcggagaaagaggtaatgaaatggcacatggtcatagctgtttcc tgacccagctttcttgtacaaagtggtagatctgccggtctccctatagt gagtcgtattaatttcgataagccaggttaacctgcattaatgaatcggc tgcagtacccgggaatttaacccgcctaatgagcgggctttt Sequencing primers: M13(-21), M13 reverse, t7, sp6 | |||
1 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt 60 61 gacactatag aactcgaagg atccacaagt ttgtacaaaa aagcaggctt gtaaaacgac 120 121 ggccagtaac tataacggtc ctaaggtagc gaggcctggg tggcgaattc ccttactagt 180 181 ttccctcgag gcatctatgt cgggtgcgga gaaagaggta atgaaatggc acatggtcat 240 241 agctgtttcc tgacccagct ttcttgtaca aagtggtaga tctgccggtc tccctatagt 300 301 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc tgcagtaccc 360 361 gggaatttaa cccgcctaat gagcgggctt ttttttgtga tccaaaggat cttcttgaga 420 421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 480 481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 540 541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 600 601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 660 661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 720 721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 780 781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 840 841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 900 901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 960 961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgga 1020 1021 tcacaacaaa aagcccgctc attaggcggg ctaaattctc atgtttgaca gcttatcatc 1080 1081 gataagctag cggccgctag ctttaatgag ttatcgagat tttcaggagc taaggaagct 1140 1141 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa 1200 1201 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg 1260 1261 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt 1320 1321 attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc aatgaaagac 1380 1381 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact 1440 1441 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata 1500 1501 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt 1560 1561 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac 1620 1621 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa 1680 1681 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc 1740 1741 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg 1800 1801 taattggtac gtcga 1815 |
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